4EGI
Hsp90-alpha ATPase domain in complex with 2-Amino-4-ethylthio-6-methyl-1,3,5-triazine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-15 |
Detector | APEX II CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.500, 43.820, 53.810 |
Unit cell angles | 90.00, 113.87, 90.00 |
Refinement procedure
Resolution | 32.720 - 1.790 |
R-factor | 0.20408 |
Rwork | 0.202 |
R-free | 0.23584 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.930 |
Data reduction software | PROTEUM2 (suite) |
Data scaling software | PROTEUM2 (suite) |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 32.720 |
High resolution limit [Å] | 1.789 |
Rmerge | 0.031 |
Number of reflections | 21347 |
Redundancy | 7.47 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 273 | 0.2M MgCl2, 0.1M HEPE, 20%w/v PEG2000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 273K |