4EFP
Bombyx mori lipoprotein 7 isolated from its natural source at 1.33 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.211, 49.993, 55.244 |
| Unit cell angles | 93.41, 94.70, 102.78 |
Refinement procedure
| Resolution | 23.000 - 1.330 |
| R-factor | 0.18417 |
| Rwork | 0.184 |
| R-free | 0.23058 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.943 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | Auto-Rickshaw (MR-SAD protocol) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 1.380 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.109 | 0.319 |
| Number of reflections | 94674 | |
| <I/σ(I)> | 11.66 | 2.42 |
| Completeness [%] | 94.0 | 78.8 |
| Redundancy | 3.3 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES buffer, 22% PEG 3350, 200 mM KSCN, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
