4EFN

Crystal structure of H-Ras Q61L in complex with GppNHp (state 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-15
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	34.782, 82.006, 121.251
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.960 - 2.300
R-factor	0.18525
Rwork	0.183
R-free	0.23694
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4efm
RMSD bond length	0.011
RMSD bond angle	1.256
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.300	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.133	0.343
Number of reflections	8078
<I/σ(I)>	4.7	2.1
Completeness [%]	100.0	100
Redundancy	7.2	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M MES, 0.2M ammonium sulfate, 30% (w/v) PEG 5000 MME, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K