4EFM
Crystal structure of H-Ras G12V in complex with GppNHp (state 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-15 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 34.638, 81.833, 121.761 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.320 - 1.900 |
| R-factor | 0.18769 |
| Rwork | 0.186 |
| R-free | 0.22111 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4efl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.211 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.320 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.097 | 0.300 |
| Number of reflections | 14109 | |
| <I/σ(I)> | 5.4 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M MES, 0.2M ammonium sulfate, 30% (w/v) PEG 5000 MME, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
