4EFJ
Crystal structure of I-GzeII LAGLIDADG homing endonuclease in complex with DNA target site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.827, 61.178, 128.877 |
| Unit cell angles | 90.00, 116.30, 90.00 |
Refinement procedure
| Resolution | 38.510 - 2.800 |
| R-factor | 0.18756 |
| Rwork | 0.183 |
| R-free | 0.27769 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qqy |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.906 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.074 | 0.330 |
| Number of reflections | 21301 | |
| <I/σ(I)> | 22.9 | 2.76 |
| Completeness [%] | 98.7 | 90 |
| Redundancy | 6.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 30% PEG6000, 3 mM calcium chloride, 75 mM sodium chloride, 0.1 M HEPES, pH 7.5, 0.1 M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
