4EF1

Crystal structure of a pheromone cOB1 precursor/lipoprotein, YaeC family (EF2496) from Enterococcus faecalis V583 at 1.90 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-08
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1 21 1
Unit cell lengths	39.923, 96.485, 69.042
Unit cell angles	90.00, 100.55, 90.00

Refinement procedure

Resolution	29.380 - 1.900
R-factor	0.1909
Rwork	0.188
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.619
Data reduction software	XDS
Data scaling software	XSCALE (December 29, 2011)
Phasing software	PHASER (2.3.0)
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.380		1.970
High resolution limit [Å]	1.900	4.080	1.900
Rmerge	0.062	0.024	0.353
Number of reflections	39597	7503	7523
<I/σ(I)>	10.04	25.1	2.3
Completeness [%]	92.4	92.8	91.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	20.0% iso-Propanol, 20.0% PEG-4000, 0.1M Citrate pH 5.6, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K