4EEJ

Crystal Structure of the Q108K:K40L:T51V:T53C:Y19W:R58W:T29L:Q4R Mutant of Cellular Retinol Binding Protein Type II in Complex with All-trans-Retinal at 1.5 Angstrom Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	200
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.1272
Spacegroup name	P 1
Unit cell lengths	30.918, 35.756, 64.376
Unit cell angles	86.40, 86.44, 64.94

Refinement procedure

Resolution	32.360 - 1.501
R-factor	0.17262
Rwork	0.171
R-free	0.20798
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rcq
RMSD bond length	0.027
RMSD bond angle	2.389
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.360
High resolution limit [Å]	1.501
Number of reflections	39769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION	4.6	298	40% PEG4000, 0.1 M sodium acetate trihydrate, pH 4.6, 0.1 M ammonium acetate, EVAPORATION, RECRYSTALLIZATION, temperature 298K