4EDP
1.85 Angstrom resolution crystal structure of an ABC transporter from Clostridium perfringens ATCC 13124
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.318, 50.743, 143.774 |
| Unit cell angles | 90.00, 98.83, 90.00 |
Refinement procedure
| Resolution | 29.100 - 1.850 |
| R-factor | 0.16426 |
| Rwork | 0.163 |
| R-free | 0.19090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a99 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.471 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.076 | 0.573 |
| Number of reflections | 68597 | |
| <I/σ(I)> | 17.17 | 2.8 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 4.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | crystallization condition: 2M NH4 Citrate pH 7.0,0.1MBis-Tris Propane. Paratone used for cryoprotection, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
