4EDJ
Crystal structure of the GRASP55 GRASP Domain with a phosphomimetic mutation (S189D)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-29 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54148 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.857, 82.547, 84.059 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.900 - 1.901 |
| R-factor | 0.21164 |
| Rwork | 0.209 |
| R-free | 0.27052 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rle |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.927 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 58.900 | 2.580 | 1.970 |
| High resolution limit [Å] | 1.900 | 2.390 | 1.900 |
| Number of reflections | 34613 | ||
| <I/σ(I)> | 22.2 | 2.74 | |
| Completeness [%] | 98.0 | 99.9 | 99.9 |
| Redundancy | 5.9 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 0.2M ammonium acetate, 0.1M Tris pH 9.0, 0.1M immidazole, 30% PEG 4000, 10% PEG 20,000, 10% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
