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4ED0

Human DNA polymerase eta - DNA ternary complex: AT crystal at pH 6.8 (Na+ MES) with 1 Ca2+ ion

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-BM
Synchrotron siteAPS
Beamline22-BM
Temperature [K]95
Detector technologyCCD
Collection date2011-07-23
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)1
Spacegroup nameP 61
Unit cell lengths98.786, 98.786, 82.431
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution28.517 - 1.653
R-factor0.1714
Rwork0.170
R-free0.20790
Structure solution methodFOURIER SYNTHESIS
RMSD bond length0.007
RMSD bond angle1.146
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Refinement softwarePHENIX ((phenix.refine: 1.6.1_357))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.680
High resolution limit [Å]1.6501.650
Number of reflections54218
<I/σ(I)>17.91.8
Completeness [%]99.192
Redundancy3.72.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP62930.1M MES, 6-17%(W/V) PEG 2K-MME, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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