4ECF
Crystal structure of an ABC-type phosphate transport system, periplasmic component (LVIS_0633) from Lactobacillus brevis ATCC 367 at 1.55 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9116,0.9795,0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.204, 43.752, 71.475 |
| Unit cell angles | 90.00, 102.57, 90.00 |
Refinement procedure
| Resolution | 27.274 - 1.550 |
| R-factor | 0.1639 |
| Rwork | 0.162 |
| R-free | 0.19590 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.555 |
| Data reduction software | XDS |
| Data scaling software | XSCALE (December 29, 2011) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.274 | 1.610 | |
| High resolution limit [Å] | 1.550 | 3.330 | 1.550 |
| Rmerge | 0.046 | 0.028 | 0.444 |
| Number of reflections | 38657 | 7363 | 6819 |
| <I/σ(I)> | 9.06 | 20.9 | 1.8 |
| Completeness [%] | 88.6 | 92.1 | 80.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M lithium acetate, 20.0% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
