4EC9
Crystal structure of full-length cdk9 in complex with cyclin t
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.93300 |
| Spacegroup name | H 3 |
| Unit cell lengths | 174.229, 174.229, 97.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.600 - 3.210 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.23300 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.456 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 3.380 |
| High resolution limit [Å] | 3.208 | 3.210 |
| Rmerge | 0.514 | |
| Number of reflections | 17982 | |
| <I/σ(I)> | 11.7 | 1.5 |
| Completeness [%] | 98.9 | 98.2 |
| Redundancy | 2.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 277 | 1% PEG 1K, 200MM NK PHSOPHATE, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
