4EC6
Ntf2-like, potential transfer protein TraM from Gram-positive conjugative plasmid pIP501
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.210, 54.980, 93.470 |
| Unit cell angles | 89.91, 86.44, 78.63 |
Refinement procedure
| Resolution | 46.890 - 2.500 |
| R-factor | 0.2118 |
| Rwork | 0.209 |
| R-free | 0.26109 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.762 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE (2.13) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 10.800 | 2.500 |
| Rmerge | 0.116 | 0.026 | 0.480 |
| Number of reflections | 25885 | ||
| <I/σ(I)> | 14.91 | 51.73 | 4.05 |
| Completeness [%] | 97.5 | 98.8 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 7.33 | 298 | 1 mg/ml protein, 16.5% PEG 3350 (final concentration in drop v/v), 0.1 M Hepes, pH 7.33, microbatch under oil, temperature 298K |
