4EC5
Crystal structure of the S210C (dimer) mutant from the N-terminal domain of the secretin XcpQ from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Wavelength(s) | 0.8726 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.790, 39.780, 93.900 |
| Unit cell angles | 90.00, 99.75, 90.00 |
Refinement procedure
| Resolution | 46.602 - 2.197 |
| R-factor | 0.2141 |
| Rwork | 0.213 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.165 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.197 |
| Number of reflections | 22248 |
| Completeness [%] | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.1M HEPES pH 7.5, 14% PEG8000, 0.2M Calcium acetate hydrate, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
