4EBY

Crystal structure of the ectodomain of a receptor like kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	298
Detector technology	CCD
Collection date	2011-03-15
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	56.829, 71.128, 72.761
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.213 - 1.650
R-factor	0.1718
Rwork	0.171
R-free	0.18900
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.281
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: dev_596))

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000
High resolution limit [Å]	1.642
Number of reflections	35917
Completeness [%]	96.9
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1M Sodium Cacodylate pH6.5, 0.2M sodium acetate, 30% (v/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K