4EBJ
Crystal structure of aminoglycoside 4'-O-adenylyltransferase ANT(4')-IIb, apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.444, 83.482, 90.166 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.963 - 1.600 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.19190 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.057 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 154555 | |
| <I/σ(I)> | 27.31 | 2.49 |
| Completeness [%] | 99.6 | 97.6 |
| Redundancy | 5.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2 M ammonium sulfate, 0.1 M MES, 30% PEG 5K MME, cryoprotectant: 25% ethylene glycol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
