4E9J
Crystal structure of the N-terminal domain of the secretin XcpQ from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Wavelength(s) | 0.9193 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.500, 39.720, 93.300 |
| Unit cell angles | 90.00, 99.84, 90.00 |
Refinement procedure
| Resolution | 45.960 - 2.030 |
| R-factor | 0.2123 |
| Rwork | 0.210 |
| R-free | 0.24890 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (+ BUSTER 2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.670 |
| High resolution limit [Å] | 2.030 |
| Number of reflections | 28407 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 293.15 | 0.2M MgCl2, 0.1M Tris pH 8.5, 25% PEG3350, EVAPORATION, temperature 293.15K |
