4E9J
Crystal structure of the N-terminal domain of the secretin XcpQ from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Wavelength(s) | 0.9193 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.500, 39.720, 93.300 |
Unit cell angles | 90.00, 99.84, 90.00 |
Refinement procedure
Resolution | 45.960 - 2.030 |
R-factor | 0.2123 |
Rwork | 0.210 |
R-free | 0.24890 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.160 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (+ BUSTER 2.10.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.670 |
High resolution limit [Å] | 2.030 |
Number of reflections | 28407 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.5 | 293.15 | 0.2M MgCl2, 0.1M Tris pH 8.5, 25% PEG3350, EVAPORATION, temperature 293.15K |