4E99
Human Serum Albumin Complex with Perfluorooctane Sulfonate Potassium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9321 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.466, 83.756, 56.004 |
| Unit cell angles | 90.00, 103.50, 90.00 |
Refinement procedure
| Resolution | 54.460 - 2.300 |
| R-factor | 0.24015 |
| Rwork | 0.238 |
| R-free | 0.27789 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lu8 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.862 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 21658 | |
| Completeness [%] | 99.5 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION, RECRYSTALLIZATION | 7.5 | 298 | 30-36% PEG3350, 50mM potassium phosphate, pH 7.5, EVAPORATION, RECRYSTALLIZATION, temperature 298K |
