4E8T
Structure of Oceanobacillus iheyensis group II intron in the presence of K+, Ca2+ and an oligonucleotide fragment substrate (low energy dataset)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.6083 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.458, 95.765, 225.688 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.610 - 3.340 |
| R-factor | 0.19129 |
| Rwork | 0.189 |
| R-free | 0.23544 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3igi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 2.302 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.612 | 3.420 |
| High resolution limit [Å] | 3.340 | 3.340 |
| Rmerge | 0.085 | 0.754 |
| Number of reflections | 27121 | |
| <I/σ(I)> | 13.4 | 2 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 100 mM potassium acetate, 100 mM potassium chloride, 100 mM calcium chloride, 50 mM HEPES sodium, pH 7.0, 5% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
