4E8P
Structure of Oceanobacillus iheyensis group II intron in a ligand-free state in the presence of Rb+ and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.8148 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.987, 95.024, 223.492 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.160 - 3.280 |
| R-factor | 0.19137 |
| Rwork | 0.189 |
| R-free | 0.24373 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3igi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 2.469 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.164 | 3.360 |
| High resolution limit [Å] | 3.280 | 3.280 |
| Rmerge | 0.076 | 0.737 |
| Number of reflections | 27907 | |
| <I/σ(I)> | 11.1 | 1.8 |
| Completeness [%] | 99.1 | 99.6 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 100 mM magnesium acetate, 150 mM rubidium chloride, 50 mM HEPES sodium, pH 7.0, 4% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
