4E8D
Crystal structure of streptococcal beta-galactosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.971, 79.300, 99.387 |
| Unit cell angles | 90.00, 106.94, 90.00 |
Refinement procedure
| Resolution | 46.980 - 1.800 |
| R-factor | 0.17556 |
| Rwork | 0.174 |
| R-free | 0.20996 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d3a |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.929 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | |
| Number of reflections | 108735 | |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 98.8 | 98.8 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
