4E85

crystal STRUCTURE OF HAT DOMAIN OF RNA14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	114.870, 241.730, 49.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.770 - 3.000
R-factor	0.233
Rwork	0.233
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4e6h
RMSD bond length	0.014
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	COMO
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.106	0.378
Number of reflections	26572
<I/σ(I)>	8.6742	2.44
Completeness [%]	92.5	67.3
Redundancy	3.6	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	50 mM HEPES 7.5, 13% PEG 3350, 1% tryptone, VAPOR DIFFUSION, SITTING DROP, temperature 293K