4E84

Crystal Structure of Burkholderia cenocepacia HldA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-24
Detector	ADSC QUANTUM 210
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	90.398, 90.398, 397.917
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.955 - 2.600
R-factor	0.2264
Rwork	0.224
R-free	0.26320
RMSD bond length	0.006
RMSD bond angle	0.695
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.960	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	31023
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2M sodium citrate, 0.1M HEPES, 20% 2-propanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K