4E7V
The structure of R6 bovine insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-17 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0400 |
| Spacegroup name | H 3 |
| Unit cell lengths | 156.240, 156.240, 78.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.880 - 1.800 |
| R-factor | 0.2119 |
| Rwork | 0.209 |
| R-free | 0.27170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ev3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.982 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 2010_07_29_2140)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.880 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 64897 |
| Completeness [%] | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 298 | 2 ul 5 mg/ml bovine insulin + 2 ul reservoir equilibrated against 1 ml reservoir of: 0.12 M sodium citrate, 17 mM zinc acetate, 22% acetone, 0.5% m-cresol, 1.67 M NaCl, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
