4E7T
The structure of T6 bovine insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-17 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0400 |
| Spacegroup name | H 3 |
| Unit cell lengths | 80.980, 80.980, 33.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.780 - 1.400 |
| R-factor | 0.1956 |
| Rwork | 0.194 |
| R-free | 0.22850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mso |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.449 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 2010_07_29_2140)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.780 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 15790 |
| Completeness [%] | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 0.05M sodium citrate, 0.5mM zinc acetate, 15% acetone, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
