4E6Z
Tic22 from Plasmodium falciparum
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9800, 0.9802, 0.9537 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 66.250, 66.250, 129.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.283 - 2.150 |
R-factor | 0.2178 |
Rwork | 0.217 |
R-free | 0.24190 |
Structure solution method | MAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.634 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.283 | 2.270 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.100 | 0.627 |
Number of reflections | 15234 | |
<I/σ(I)> | 7.9 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.2 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 288 | 0.1M MES pH 6.0, 20% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 288K |