4E6S

Crystal structure of the SCAN domain from mouse Zfp206

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.081
Spacegroup name	I 4 2 2
Unit cell lengths	67.565, 67.565, 87.544
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.776 - 1.850
R-factor	0.2068
Rwork	0.204
R-free	0.23510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.984
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.045	0.468
Number of reflections	8995
<I/σ(I)>	47.538	5.976
Completeness [%]	100.0	100
Redundancy	15.2	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.3M ammonium sulfate, 0.1 M Tris-Cl, pH 8.6, 25% PEG 3350, 0.1M EDTA, VAPOR DIFFUSION, HANGING DROP, temperature 291K
2	VAPOR DIFFUSION, HANGING DROP	8	291	0.3M ammonium sulfate, 0.1 M Tris-Cl, pH 8.6, 25% PEG 3350, 0.1M EDTA, Mercury derivative, VAPOR DIFFUSION, HANGING DROP, temperature 291K