4E6R
Crystal structure of a Cytoplasmic protein NCK2 (NCK2) from Homo sapiens at 2.20 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-12 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97949,0.97929 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 108.398, 108.398, 68.199 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.862 - 2.200 |
| R-factor | 0.2024 |
| Rwork | 0.201 |
| R-free | 0.22690 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.769 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.862 | 28.862 | 2.260 |
| High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
| Rmerge | 0.021 | 0.021 | |
| Total number of observations | 1865 | 12870 | |
| Number of reflections | 12480 | ||
| <I/σ(I)> | 11.4 | 10.8 | 0.3 |
| Completeness [%] | 100.0 | 96.2 | 100 |
| Redundancy | 14 | 10.8 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 40.00% polyethylene glycol 300, 0.20M zinc acetate, 0.1M Imidazole pH 8.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
