4E6N
Crystal structure of bacterial Pnkp-C/Hen1-N heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.630, 59.695, 101.540 |
| Unit cell angles | 81.38, 87.82, 88.82 |
Refinement procedure
| Resolution | 34.645 - 2.390 |
| R-factor | 0.1699 |
| Rwork | 0.168 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.082 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.390 |
| Number of reflections | 49244 |
| Completeness [%] | 89.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.75 | 303 | 6% PEG6000, 0.5M NACL, 25MM CACL2, 5% METHANOL, 50MM HEPES, 50 MM TRIS-HCL, pH 7.75, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
