4E61

Crystal structure of the EB1-like motif of Bim1p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.254330
Spacegroup name	P 1 21 1
Unit cell lengths	28.000, 42.500, 100.500
Unit cell angles	90.00, 90.30, 90.00

Refinement procedure

Resolution	20.000 - 2.450
Rwork	0.211
R-free	0.24110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Not fully refined model after structure determination by SAD with potassium tetranitroplatinate(II)-soaked crystals.
RMSD bond length	0.098
RMSD bond angle	1.180
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	BUSTER-TNT (2.X)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.450	2.450
Number of reflections	8832
Completeness [%]	98.8	98.29

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294.15	0.1M Bis-Tris pH 8.0, 20% PEG 5000MME, 40% gamma-butyrlactone, VAPOR DIFFUSION, HANGING DROP, temperature 294.15K