4E5C
Crystal Structure of 19mer double-helical RNA containing CUG/CGG-repeats
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9724 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 45.154, 45.154, 104.036 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 1.700 |
R-factor | 0.214 |
Rwork | 0.212 |
R-free | 0.24440 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.865 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 1.760 |
High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
Rmerge | 0.041 | 0.036 | 0.098 |
Number of reflections | 13505 | ||
<I/σ(I)> | 21.2 | ||
Completeness [%] | 94.8 | 85.2 | 80.4 |
Redundancy | 3.2 | 3 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 1.8 mM ammonium sulfate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |