4E4C
Crystal structure of Notexin at 1.8 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.46 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.100, 74.100, 48.738 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.800 - 1.800 |
| R-factor | 0.26569 |
| Rwork | 0.263 |
| R-free | 0.32247 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ae7 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.074 | 0.377 |
| Number of reflections | 13984 | |
| Completeness [%] | 95.4 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 100 mM MES, 2.5 ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
