4E4B
Structure of the fusidic acid resistance protein FusB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.2837 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 55.370, 89.100, 107.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.030 - 2.800 |
| R-factor | 0.24451 |
| Rwork | 0.241 |
| R-free | 0.31422 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yb5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.061 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.030 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.094 |
| Number of reflections | 6519 |
| <I/σ(I)> | 9.7 |
| Completeness [%] | 99.8 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.2 M magnesium chloride, 0.1 M Tris-HCl, 45% (v/v) PEG 400, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
