4E3U

Crystal Structure of Hen Egg White Lysozyme Cryoprotected in Proline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	123
Detector technology	IMAGE PLATE
Collection date	2011-10-25
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	77.995, 77.995, 37.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.499 - 1.500
R-factor	0.1864
Rwork	0.185
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2lyz
RMSD bond length	0.005
RMSD bond angle	1.012
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.499	19.499	1.580
High resolution limit [Å]	1.500	4.750	1.500
Rmerge		0.019	0.241
Total number of observations		8320	21942
Number of reflections	19076
<I/σ(I)>	46.1	29.5	3.1
Completeness [%]	99.6	98.7	97.6
Redundancy	10.8	12	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	296	1.0M sodium chloride, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 296K