4E38
Crystal structure of probable keto-hydroxyglutarate-aldolase from Vibrionales bacterium SWAT-3 (Target EFI-502156)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.503, 80.960, 123.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.720 - 1.640 |
| R-factor | 0.17175 |
| Rwork | 0.171 |
| R-free | 0.20438 |
| Structure solution method | MR |
| Starting model (for MR) | 1vhc |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.318 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.094 | |
| Number of reflections | 92852 | |
| <I/σ(I)> | 10.1 | 2 |
| Completeness [%] | 98.9 | 86.8 |
| Redundancy | 8.1 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.1M BIS-TRIS, 0.5M MAGNESIUM FORMATE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
