4E2L
Crystal Structure of the periplasmic domain of mutant FepE LPS O-antigen chain length regulator protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-13 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.307, 141.257, 136.888 |
| Unit cell angles | 90.00, 107.03, 90.00 |
Refinement procedure
| Resolution | 49.716 - 2.800 |
| R-factor | 0.244 |
| Rwork | 0.242 |
| R-free | 0.28910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.212 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.716 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 93614 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.5M KCl, 0.05M MOPS, 12% (w/v) PEG4K, 20% w/v glycerol, pH 7.0, vapor diffusion, hanging drop, temperature 277K |
