4E27
Crystal Structure of a Pentameric Capsid Protein Isolated from Metagenomic Phage Sequences Solved by Iodide SAD Phasing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-14 |
| Detector | RIGAKU SATURN 944+ |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.710, 68.360, 107.830 |
| Unit cell angles | 90.00, 93.69, 90.00 |
Refinement procedure
| Resolution | 43.510 - 2.400 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.081 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MLPHARE |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.098 | 0.371 |
| Number of reflections | 75296 | |
| <I/σ(I)> | 14.39 | 4.88 |
| Completeness [%] | 99.5 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 20% PEG 3350, 0.2M K Formate, no buffer, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
