4E1R
Crystal structure of the dimerization domain of Lsr2 from Mycobacterium tuberculosis in the P 31 2 1 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2011-10-27 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9786 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 57.390, 57.390, 105.310 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.143 - 2.041 |
R-factor | 0.2547 |
Rwork | 0.251 |
R-free | 0.28710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e1p |
RMSD bond length | 0.008 |
RMSD bond angle | 0.997 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER (MR) |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.655 | 2.150 |
High resolution limit [Å] | 2.041 | 2.041 |
Rmerge | 0.096 | 0.562 |
Number of reflections | 13341 | |
<I/σ(I)> | 22.6 | 6.2 |
Completeness [%] | 100 | |
Redundancy | 20.4 | 20.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.01 M cobalt(II) chloride, 1.4 M ammonium sulfate, 0.1 M MES, pH 6.5, 7.5% 1,4-dioxane, 0.2% w/v trypsin, VAPOR DIFFUSION, HANGING DROP, temperature 291K |