4E1K
GlmU in complex with a Quinazoline Compound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2008-10-05 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 109.028, 109.028, 323.602 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.550 - 2.000 |
| R-factor | 0.24662 |
| Rwork | 0.245 |
| R-free | 0.28363 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.101 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.400 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.320 | |
| Number of reflections | 46480 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 92.4 | 62 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 296 | 1.6-1.8 M ammonium sulfate and 0.1 M MES pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
