4E1D
Structure of a VgrG Vibrio cholerae toxin ACD domain in complex with ADP and Mn++
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-03-04 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 128.390, 128.390, 76.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.390 - 2.490 |
R-factor | 0.223 |
Rwork | 0.222 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e1c |
RMSD bond length | 0.008 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.560 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.106 | 0.416 |
Number of reflections | 42340 | |
<I/σ(I)> | 10.2 | 3 |
Completeness [%] | 100.0 | 94.8 |
Redundancy | 4.2 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |