4E19
Crystal structure of RNase H1 from halophilic archaeon Halobacterium salinarum NRC-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-10 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 41 |
| Unit cell lengths | 115.802, 115.802, 38.282 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.940 - 1.410 |
| R-factor | 0.1734 |
| Rwork | 0.172 |
| R-free | 0.20660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ehg |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.410 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.430 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.074 | 0.381 |
| Number of reflections | 48997 | |
| <I/σ(I)> | 31.61 | 3.613 |
| Completeness [%] | 99.9 | 98.9 |
| Redundancy | 7.4 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 20%(W/V) PEG 8000, Imidazole(pH6.5), 3%(V/V) MPD, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
