4E11
Crystal structure of kynurenine formamidase from Drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0750 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.771, 47.085, 85.683 |
| Unit cell angles | 90.00, 90.34, 90.00 |
Refinement procedure
| Resolution | 42.840 - 2.000 |
| R-factor | 0.22743 |
| Rwork | 0.225 |
| R-free | 0.27344 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pbl |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.663 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.150 | 0.620 |
| Number of reflections | 19948 | |
| <I/σ(I)> | 23.3 | |
| Redundancy | 7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
