4DZS

Crystal structure of yeast Puf4p RNA binding domain in complex with HO-4BE mutant RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	65.420, 135.750, 148.411
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.283 - 3.140
R-factor	0.265
Rwork	0.260
R-free	0.31620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bwt
RMSD bond length	0.003
RMSD bond angle	0.606
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.140	3.140
Number of reflections	22435
Completeness [%]	96.1	77.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2.0 M ammonium sulfate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K