4DZP
Crystal structure of the terminase small subunit gp1 with R48A mutation of the bacterial virus sf6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2010-03-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 89.060, 89.060, 72.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.221 - 1.800 |
| R-factor | 0.2111 |
| Rwork | 0.209 |
| R-free | 0.25180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 3HEF |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.859 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.221 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.034 | 0.203 |
| Number of reflections | 27623 | |
| <I/σ(I)> | 31.71 | 7.78 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 8.38 | 8.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 1.2M NAH2PO4/0.8M K2HPO4, 0.2M Li2SO4, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
