4DZO

Structure of Human Mad1 C-terminal Domain Reveals Its Involvement in Kinetochore Targeting

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97937
Spacegroup name	P 65
Unit cell lengths	44.767, 44.767, 211.056
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.955 - 1.760
R-factor	0.1635
Rwork	0.160
R-free	0.19680
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.205
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.960	50.000	1.790
High resolution limit [Å]	1.760	4.780	1.760
Rmerge	0.081	0.055	0.676
Number of reflections	23608
<I/σ(I)>	10.2
Completeness [%]	99.9	99.3	98.6
Redundancy	14.8	14.7	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging-drop vapor diffusion	6.2	293	20 mM Tris, 100 mM KCl, 1 mM TCEP, 26% (w/v) PEG1500, 0.1 M Na Cacodylate, pH 6.2, 1mM reduced L-Glutathione;, hanging-drop vapor diffusion, temperature 293K