4DZD

Crystal structure of the CRISPR-associated protein Cas6e from Escherichia coli str. K-12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	59.900, 59.900, 128.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.376 - 1.999
R-factor	0.2042
Rwork	0.202
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wj9
RMSD bond length	0.006
RMSD bond angle	0.986
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	1.999	5.430	2.000
Rmerge	0.100	0.057	0.371
Number of reflections	16585
<I/σ(I)>		6.1	7.2
Completeness [%]	99.9	98.8	99.5
Redundancy		8.3	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	0.1M Bis-Tris, 0.2M ammonium acetate, 25% PEG3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K