4DZA
Crystal structure of a lysine racemase within internal aldimine linkage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2010-07-20 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.847, 85.086, 151.292 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.740 |
| R-factor | 0.22215 |
| Rwork | 0.220 |
| R-free | 0.26069 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dyj |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.477 |
| Data reduction software | MOSFLM |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 39932 | |
| Completeness [%] | 80.5 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1M Sodium-acetate, 0.05M Lithium-chloride, 29% PEG8000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
