4DYZ

Crystal Structure of the apo form of Human H-Ferritin variant MIC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-17
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.98
Spacegroup name	F 4 3 2
Unit cell lengths	181.066, 181.066, 181.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.540 - 2.300
R-factor	0.2231
Rwork	0.219
R-free	0.27150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cei
RMSD bond length	0.008
RMSD bond angle	0.996
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	104.538	90.533	2.420
High resolution limit [Å]	2.300	7.270	2.300
Rmerge		0.041	0.397
Total number of observations		15792	72134
Number of reflections	11848
<I/σ(I)>	36.1	14.3	1.9
Completeness [%]	100.0	99.9	100
Redundancy	42	34	42.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	50 mM Tris, 10 mM calcium chloride, 4% PEG 400, soaked in 20 mM EDTA, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K