4DYR
Crystal structure of terminase small subunit gp1 of the bacterial virus sf6 with CAPS PH10.5 buffer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2009-12-05 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 88.852, 88.852, 72.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.168 - 1.650 |
| R-factor | 0.1976 |
| Rwork | 0.196 |
| R-free | 0.22130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hef |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.899 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.042 | 0.381 |
| Number of reflections | 34923 | |
| <I/σ(I)> | 48.1 | 4.06 |
| Completeness [%] | 97.9 | 94.5 |
| Redundancy | 6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 1.2M NAH2PO4/0.8M K2HPO4, 0.2M Li2SO4, 0.1M CAPS pH 10.5, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
