4DYL
F-BAR domain of human FES tyrosine kinase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97780 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 74.224, 30.112, 181.838 |
| Unit cell angles | 90.00, 94.34, 90.00 |
Refinement procedure
| Resolution | 29.730 - 2.180 |
| R-factor | 0.21771 |
| Rwork | 0.216 |
| R-free | 0.25452 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.291 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.730 | 2.300 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.136 | 0.580 |
| Number of reflections | 21232 | |
| <I/σ(I)> | 9.3 | 2.8 |
| Completeness [%] | 98.9 | 92.8 |
| Redundancy | 5.1 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.20M Na(malonate), 0.1M BTProp pH 6.5, 20.0% PEG 3350, 10.0% Ethylene Glycol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
